Information-enriched characterization of mobile populations. Morphological information from pictures can incorporate on the richness of flow cytometry information. As an example, the figure shows an usually conventional apoptosis assay utilizing Annexin V and PI, incorporating cell imaging to characterize cells in Every inhabitants to reveal morphologically unique features. These insights couldn't are already acquired from multiplex staining by itself.
As samples are obtained over the Attune CytPix Flow Cytometer, the large-pace brightfield digicam captures and stores photographs of detected situations at a amount nearly 6,000 pictures for every 2nd, dependant upon the flow price and graphic size. For increased versatility, Attune Cytometric Software program helps you to alter the picture capture frequency as wanted and select a selected gate for picture seize, if necessary.
(B) RAW 264.7 macrophages are often annotated inaccurately with respect to particle count because of their complexity and noncircular borders. The volume of singlets accessible for Evaluation raises as soon as the consumer trains and employs a more exact product.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing which has a substantial-pace brightfield digital camera for simultaneous superior throughput flow cytometry and large resolution brightfield imaging.
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On top of that, it is actually illuminating to determine the number of doublets slip via traditional singlet gates, and wonderful to validate the position of unanticipated double-optimistic situations in my Assessment."
Pair your Attune flow cytometer using an autosampler for enhanced workflow effectiveness, and incorporate robotic integration for maximum throughput.
Our scientists will style panels for yourself as over and over you'll need—free of charge, no products buy necessary.
Early signs of this contamination experienced originally been dismissed as particles. Backgating to find out new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also enables you to use morphological options to find appealing subpopulations that could not be apparent from flow cytometry information by yourself.
Customers can perspective function visuals in actual-time to confirm sample excellent, enhance protocols, and document the morphology of every cell inhabitants determined for Examination. Imaging capture also benefits from acoustic focusing, which helps you to place the cells to make sure that a sharp, centered impression is attained.
Seem waves allow exact mobile alignment, resulting in Fantastic facts high quality and ~10X more rapidly sample flow prices.
Software Notice: Automated graphic Investigation decreases person-to-user variability in flow cytometry gating tactics
Leverage the power of advanced technologies and innovation to accelerate your discoveries. Believe in while in the Attune Flow Cytometer relatives to deliver the dependability, velocity, and precision that set your operate apart.
No clean/no lyse leukocyte Examination with imaging. Cells were being obtained from 24-hour-aged blood by dilution in 1 mM EDTA ( Professional UX/UI enables consumers to train AI styles for read more expanded apps. (A) The “Educate Product” function supplies annotation tools and authentic-time model training accuracy.
Gating on these extended parameters allows you to promptly and precisely establish populations of interest and confirm gating system with little or no handbook review. The Attune software program delivers access to state-of-the-artwork equipment Studying Examination tools within just an expertly developed consumer interface, rendering it extremely user friendly.
The Attune Xenith cytometer is usually a large-parameter spectral analyzer, furnishing each spectral unmixing and traditional flow capabilities for detailed and functional mobile analysis.
Agilent Worth Assure guarantees you no less than 10 years of instrument use within the date you purchase. In any other case, We're going to credit rating you Together with the residual worth of the method toward an upgraded model.
What gave the impression to be a homogeneous population is in fact a lot more varied—an insight that ought to be viewed as when interpreting outcomes.